Table 6.
Anti-HLA-E mAbs Reported To Be “Specific” for HLA-E (MEM-E/02 and 3D12) Cross-React with Several HLA-Ia Molecules
| HLA alleles | MEM-E/02 | 3D12 | HLA alleles | MEM-E/02 | 3D12 | HLA alleles | MEM-E/02 | 3D12 |
|---|---|---|---|---|---|---|---|---|
| A*1101 (A11) | 5171 | 2372 | B*1510 (B71) | 1566 | B*5101 (B51) | 3518 | ||
| A*2402 (A24) | 2072 | B*1511 (B75) | 4917 | B*5102 (B51) | 1915 | |||
| A*2403 (A24) | 1034 | B*1513 (B77) | 3738 | B*5201 (B52) | 4137 | |||
| A*2601 (A26) | 2873 | B*1516 (B63) | 1451 | B*5301 (B53) | 6999 | 2960 | ||
| A*2901 (A29) | 1319 | B*1801 (B18) | 2996 | B*5401 (B54) | 3784 | |||
| A*3001 (A30) | 1013 | B*2708 (B27) | 1012 | B*5501 (B55) | 3980 | |||
| A*3002 (A30) | 1272 | B*3501 (B35) | 2219 | B*5601 (B56) | 2014 | |||
| A*3203 (A32) | 1204 | B*3701 (B37) | 7514 | B*5701 (B57) | 1724 | |||
| A*3301 (A33) | 1575 | B*3801 (B38) | 1325 | B*5703 (B57) | 2193 | 1032 | ||
| A*3301 (A33) | 1561 | B*4001 (B60) | 4912 | 1328 | B*5801 (B58) | 5683 | 3126 | |
| A*3401 (A34) | 1950 | B*4002 (B61) | 2771 | B*5901 (B59) | 5112 | 1303 | ||
| A*4301 (A43) | 1010 | B*4006 (B61) | 9540 | 4899 | B*7301 (B73) | 2255 | ||
| A*8001 (A80) | 1161 | B*4101 (B41) | 4040 | B*7801 (B78) | 2947 | |||
| B*0801 (B08) | 1858 | B*4402 (B44) | 5330 | B*8101 (B81) | 1898 | |||
| B*1301 (B13) | 5811 | 1720 | B*4403 (B44) | 4010 | 1011 | B*8201 (B82) | 5700 | |
| B*1302 (B13) | 4699 | 1118 | B*4501 (B45) | 1691 | ||||
| B*1401 (B64) | 5653 | 1677 | B*4601 (B46) | 2652 | ||||
| B*1402 (B65) | 2624 | B*4701 (B47) | 2684 | Both mAbs failed to react | ||||
| B*1502 (B75) | 2675 | B*4801 (B48) | 6294 | With iBeads containing only | ||||
| B*1503 (B72) | 5619 | B*4901 (B49) | 1388 | With β2aHC (MFI <1000). | ||||
The mAbs were diluted 1/100. Only those alleles with MFI above >1000 are presented. The mAbs were tested on Luminex SABs assay using regular LABSCreen beadsets (Lot-7) and with iBeads. The regular beadsets are coated with both β2m-associated, peptide–associated, or peptide-free heavy chain (β2aHC) with admixture of β2m-free heavy chains of HLA (β2fHC).(63)
β2m, β2-microglobulin; MFI, mean fluorescent intensity; SABs, single antigen beads.