Table 7.

HLA-E Heavy Chain Monomer, Upon Immunization in a Mouse Model, Elicits Eight Different Groups of Anti-HLA-E Antibodies; However, Only Group 1 Befits the Definition of HLA-E-Specific Monoclonal Antibody Because of Its Monospecificity

Classification of the anti-HLA-E mAbs generated at TFL using heavy chain of HLA-E
Anti-HLA mAbs	Classical HLA (HLA-Ia)			Nonclassical HLA (HLA-Ib)			Characteristics of the groups of mAbs
Alleles ->	A	B	Cw	E	F	G
Group 1	0	0	0	+	0	0	HLA-E monospecific
Group 2	0	0	0	+	+	0	HLA-F cross-reactive
Group 3	0	0	0	+	0	+	HLA-G cross-reactive
Group 4	0	0	0	+	+	+	HLA-Ib specific
Group 5	+	+	+	+	0	0	HLA-Ia polyreactive
Group 6	+	+	+	+	+	0	HLA-Ia/F polyreactive
Group 7	+	+	+	+	0	+	HLA-Ia/G polyreactive
Group 8	+	+	+	+	+	+	HLA class-I specific

The monospecific monoclonal HLA-E antibodies recognize none of the other HLA-Ia or Ib molecules. TFL refers to Terasaki Foundation Laboratory in Los Angeles, where the antibodies were generated and characterized. The mAbs were monitored in Luminex SABs assay using regular LABSCreen beadsets.