Skip to main content
. 2019 Jul 16;14(7):e0218990. doi: 10.1371/journal.pone.0218990

Table 4. α-chain TCR transcripts (CDR3 region) expressed in aneurysmal wall of patients with AAA.

Clone Vα N Jα Transcript Frequency in Specimen p value
PATIENT AAA03
α-chain TCR transcripts amplified by NPA- PCR/Vα-specific PCR vs.
1/57 		vs.
2/57
aaa03npa04 C A L E G Y S S Vα5.1Jα3 p = 0.003 p = 0.034
tgtgct ctaga ggggtacagcagt 5/57(9%)
aaa03npa21 C V V S A G R E Y G N K L Vα24.1Jα47 p = 0.003 p = 0.034
tgtgtggtgagc gcgggtag ggaatatggaaacaaactg 5/57(9%)
aaa03npa09 C A Y I S G N K L Vα14.1Jα47 p = 0.015 p = 0.089
tgtgcttat atctc tggaaacaaactg 4/57(7%)
aaa03npa33 C A M R E G E G G S N Y K L Vα6.1Jα54 p = 0.015 p = 0.089
tgtgcaatgaga gagggcgag ggaggtagcaactataaactg 4/57(7%)
aaa03npa16 C A V E E T S G S R L Vα13.1Jα58 p = 0.06 p = 0.183
tgtgctgtg g aagaaaccagtggctctaggttg 3/57(5%)
aaa03npa02 C A G Q K G G T S Y G K L Vα25.1Jα53 p = 0.06 p = 0.183
tgtgctggg cagaag ggtggtactagctatggaaagctg 3/57(5%)
aaa03npa19 C A V E T G F Q K L Vα11.1Jα8 ns ns
tgtgctgtggag acc ggctttcagaaactt 2/57(4%)
aaa03npa12 C A V V R N N N D R L Vα13.1Jα31 ns ns
tgtgctgtc gttc ggaataacaatgacagactc 2/57(4%)
aaa03npa10 C A V V T S V G S G N T G K L Vα13.1Jα37 ns ns
tgtgctgtg gtcactagcgt tggctctggcaacacaggcaaacta 2/57(4%)
aaa03npa26 C A V A K D G G S Q G N L Vα13.1Jα42 ns ns
tgtgctgtg gcaaagg atggaggaagccaaggaaatctc 2/57(4%)
aaa03npa11 C A V E G S S G D K L Vα13.1Jα46 ns ns
tgtgctgtg gaggg aagcagcggagacaagctg 2/57(4%)
aaa03npa20 S A V Y P S P V A G G G N K L Vα21.1Jα10 ns ns
tctgcagtgtac ccatctcccgtcg cgggaggaggaaacaaactc 2/57(4%)
aaa03npa27 C A T D S F V Y S N A S K I Vα3.1Jα3 ns ns
tgtgctacg gactctttcgtttacagcaa tgcttccaagata 1/57(2%)
aaa03npa61 C A T A R M D S S Y K L Vα3.1Jα12 ns ns
tgtgctacg gccc ggatggatagcagctataaattg 1/57(2%)
aaa03npa36 C A T D A N D Y K L Vα3.1Jα20 ns ns
tgtgctacg gacgca aacgactacaagctc 1/57(2%)
Remaining 18 of 57 sequences are unique when compared to each other and are shown in the supporting information section, S2 Table.
α-chain TCR transcripts amplified by single Vα5-specific PCR vs.
1/20		 vs.
2/20
aaa03npa04 C A L E G Y S S A S K I Vα5.1Jα3 p<0.0001 p<0.0001
tgtgct ctaga ggggtacagcagtgcttccaagata 20/20(100%)
α-chain TCR transcripts amplified by single Vα24-specific PCR vs.
1/22		 vs.
2/22
aaa03npa21 C V V S A G R E Y G N K L Vα24.1Jα47 p<0.0001 p<0.0001
tgtgtggtgagc gcgggtag ggaatatggaaacaaactg 13/22(59%)
aaa03va2401 C V V S S G G S Y I Vα24.1Jα6 p = 0.0003 p = 0.0092
tgtgtggtgagc tcaggaggaagctacata 6/22(27%)
aaa03va2408 C V A A T G G F K T Vα24.1Jα9 p = 0.06 p = 0.189
tgtgtg gcggc tactggaggcttcaaaact 3/22(14%)
PATIENT AAA09
α-chain TCR transcripts amplified by NPA-PCR/Vα-specific PCR vs.
1/36		 vs.
2/36
aaa09npa08 C A L S E P F Q T G A N N L Vα12.1Jα36 p = 0.06 p = 0.187
tgtgctctgagt gagccttt tcaaactggggcaaacaacctc 3/36(8%)
aaa09npa16 C A L S R G S Q G N L Vα9.1Jα42 p = 0.06 p = 0.187
tgtgctctg tcta gaggaagccaaggaaatctc 3/36(8%)
aaa09npa01 C A M R E G D Q A G T A L Vα6.1Jα15 ns ns
tgtgcaatgaga gagggag accaggcaggaactgctctg 2/36(8%)
aaa09npa37 C A M R E I R T S Y D K V Vα6.1Jα50 ns ns
tgtgcaatgaga gagattcgg acctcctacgacaaggtg 2/36(6%)
aaa09npa22 C A L R G L N Q A G T A L Vα9.1Jα15 ns ns
tgtgctcta aggggtctc aaccaggcaggaactgctctg 2/36(6%)
aaa09npa39 C A L A P S G N T G K L Vα5.1Jα37 ns ns
tgtgctcta gcccc ctctggcaacacaggcaaacta 1/36(3%)
aaa09npa26 C A M R S N T G F Q K L Vα6.1Jα8 ns ns
tgtgcaatgaga tc gaacacaggctttcagaaactt 1/36(3%)
aaa09npa09 C A M R E G M A G F Q K L Vα6.1Jα8 ns ns
tgtgcaatgaga gagggcatgg caggctttcagaaactt 1/36(3%)
Remaining 21 of 36 sequences are unique when compared to each other and are shown in the supporting information section, S3 Table.
α-chain TCR transcripts amplified by single Vα12-specific PCR vs.
1/20		 vs.
2/20
aaa09npa08 C A L S E P F Q T G A N N L Vα12.1Jα36 p<0.0001 p<0.0001
tgtgctctgagt gagccttt tcaaactggggcaaacaacctc 11/20(55%)
aaa09va1201 C A L R G T N D Y K L Vα12.1Jα20 ns ns
tgtgctctg aggggga ctaacgactacaagctc 2/20(10%)
aaa09va1207 C A L S A G A S S S A S K I Vα12.1Jα3 ns ns
tgtgctctgagt gcaggcgcgtc cagcagtgcttccaagata 1/20(5%)
aaa09va1219 C A L S R G G N N R L Vα12.1Jα7 ns ns
tgtgctctgagt cgtgg tgggaacaacagactc 1/20(5%)
Remaining 5 of 20 sequences are unique when compared to each other and are shown in the supporting information section, S3 Table.
α-chain TCR transcripts amplified by single Vα6-specific PCR vs.
1/21		 vs.
2/21
aaa09npa37 C A M R E I R T S Y D K V Vα6.1Jα50 p = 0.06 ns
tgtgcaatgaga gagattcgg acctcctacgacaaggtg 3/21(14%)
aaa09va0609 C A M R E G L Y G G A T N K L Vα6.1Jα32 ns ns
tgtgcaatgaga gagggcctc tatggaggtgctacaaacaagctc 2/21(10%)
aaa09va0602 C A M R G G F G N V L Vα6.1Jα35 ns ns
tgtgcaatgaga ggg ggctttgggaatgtgctg 2/21(10%)
aaa09va0612 C A M R E G P G Y S S A S K I Vα6.1Jα3 ns ns
tgtgcaatgaga gagggccct gggtacagcagtgcttccaagata 1/21(5%)
aaa09va0608 C A M S P N R G G Y N K L Vα6.1Jα4 ns ns
tgtgcaatg agccctaacagg ggtggctacaataagctg 1/21(5%)
aaa09va0607 C A M R V N Y R A R R A L Vα6.1Jα5 ns ns
tgtgcaatgaga gtaaactatcggg cgggcaggagagcactt 1/21(5%)
Remaining 11 of 21 sequences are unique when compared to each other and are shown in the supporting information section, S3 Table.
α-chain TCR transcripts amplified by single Vα9-specific PCR vs.
1/19		 vs.
2/19
aaa09npa16 C A L S R G S Q G N L Vα9.1Jα42 p = 0.06 ns
tgtgctctg tcta gaggaagccaaggaaatctc 3/19(16%)
aaa09npa12 C A L R F R A Y S S A S K I Vα9.1Jα3 ns ns
tgtgctctg cgattccgtgcc tacagcagtgcttccaagata 2/19(11%)
aaa09va0906 C A L S G S G N T G K L Vα9.1Jα37 ns ns
tgtgctcta a gtggctctggcaacacaggcaaacta 2/19(11%)
aaa09npa24 C A L S R S G T R L Vα9.1Jα58 ns ns
tgtgctctaagt cggtccggga ctaggttg 2/19(11%)
aaa09va0902 C A L S G D N F N K F Vα9.1Jα21 ns ns
tgtgctctaagt gggg acaacttcaacaaattt 1/19(5%)
aaa09npa42 C A Q S P G Y N K L Vα9.1Jα4 ns ns
tgtgct cagtccc ctggtggctacaataagctg 1/19(5%)
aaa09va0905 S A S E G H G G S Q G N L Vα9.1Jα42 ns ns
tcagcc tcagaaggtc atggaggaagccaaggaaatctc 1/19(5%)
Remaining 7 of 19 sequences are unique when compared to each other and are shown in the supporting information section, S3 Table.
PATIENT AAA00
α-chain TCR transcripts following Vα2-specific PCR vs.
1/15		 vs.
2/15
aaa00va0201 C V V T G T G G F K T Vα2.3Jα9 p<0.0001 p<0.0001
tgtgtggtg acggga actggaggcttcaaaact 15/15 (100%)

α-chain TCR transcripts following Vα8-specific PCR 		vs.
1/15		 vs.
2/15
aaa00va0801 C A E E G G S N Y K L Vα8.2Jα54 p<0.0001 p<0.0001
tgtgcagag gag ggaggtagcaactataaactg 15/15 (100%)
PATIENT AAA10
α-chain TCR transcripts amplified by NPA-PCR/Vα-specific PCR vs.
1/30		 vs.
2/30
aaa10npa03 C A M T P P G G G T S Y G K L Vα6.1Jα53 p = 0.06 ns
tgtgcaatg acacctcccggg ggtggtactagctatggaaagctg 3/30(10%)
aaa10npa04 C A M R E T N T D K L Vα6.1Jα34 ns ns
tgtgcaatg agagaaacg aacaccgacaagctc 2/30(7%)
aaa10npa07 C A P F G G S N S G Y A L Vα13.1Jα41 ns ns
tgtgct cccttcggcggg tcaaattccgggtatgcactc 2/30(7%)
aaa10npa022 C G A D Y P S G T Y K Y Vα26.1Jα40 ns ns
tgtggagca gactatc cctcaggaacctacaaatac 2/30(7%)
aaa10npa09 C A T D Y S G S A R Q L Vα3.1Jα22 ns ns
tgtgctacg gactac tctggttctgcaaggcaactg 1/30(3%)
aaa10npa44 C A T P K S G T Y K Y Vα3.1Jα40 ns ns
tgtgctacc cctaag tcaggaacctacaaatac 1/30(3%)
aaa10npa43 C A T D A R H S N S G Y A L Vα3.1Jα41 ns ns
tgtgctacg gacgcccgcc actcaaattccgggtatgcactc 1/30(3%)
Remaining 18 of 30 sequences are unique when compared to each other and are shown in the supporting information section, S4 Table.
α-chain TCR transcripts following Vα6-specific PCR vs.
1/21		 vs.
2/21
aaa10npa03 C A M T P P G G G T S Y G K L Vα6.1Jα53 p = 0.01 p = 0.09
tgtgcaatg acacctcccggg ggtggtactagctatggaaagctg 4/21(19%)
aaa10npa29 C A M R E A Y S G N Q F Y Vα6.1Jα49 p = 0.01 p = 0.09
tgtgcaatgaga gaggcgtact ccggtaaccagttctat 4/21(19%)
aaa10va0601 C A M R E V D T G G F K T Vα6.1Jα9 ns ns
tgtgcaatgaga gaggtcg atactggaggcttcaaaact 2/21(10%)
aaa10va0610 C A M R E T N T D K L Vα6.1Jα34 ns ns
tgtgcaatgaga gaaacg aacaccgacaagctc 2/21(10%)
aaa10va0614 C A M R P R S G Y S T L Vα6.1Jα11 ns ns
tgtgcaatgaga ccgag gaattcaggatacagcaccctc 2/21(10%)
aaa10va0620 C A M S P M D S S Y K L Vα6.1Jα12 ns ns
tgtgcaatg agtcc gatggatagcagctataaattg 1/21(5%)
aaa10va0618 C A M R E A L M D S S Y K L Vα6.1Jα12 ns ns
tgtgcaatgaga gaggccctt atggatagcagctataaattg 1/21(5%)
aaa10va0602 C A M R D G Q K L Vα6.1Jα15 ns ns
tgtgcaatgaga ga tggccagaagctg 1/21(5%)
Remaining 4 of 21 sequences are unique when compared to each other and are shown in the supporting information section, S4 Table.
PATIENT AAA12
α-chain TCR transcripts amplified by NPA-PCR/Vα-specific PCR vs.
1/31		 vs.
2/31
aaa12npa26 C A V S A K N N N A R L Vα13.1Jα31 ns ns
tgtgctgtg tctgcgaa gaataacaatgccagactc 2/31(6%)
aaa12npa02 C A P E G G G G A D G L Vα13.1Jα45 ns ns
tgtgcc ccggagggg ggaggaggtgctgacggactc 2/31(6%)
aaa12npa16 C G A H S N S G G G A D G L Vα26.1Jα45 ns ns
tgtggagcc cactcaa attcaggaggaggtgctgacggactc 2/31(6%)
aaa12npa07 C A L R N Q T G T A L Vα5.1Jα15 ns ns
tgtgctctc cgt aaccagacaggaactgctctg 1/31(3%)
aaa12npa04 C A L E G D N A G N M L Vα5.1Jα39 ns ns
tgtgctcta gaagggg ataatgcaggcaacatgctc 1/31(3%)
aaa12npa28 C A M T S K T I I F G Q Vα6.1Jα37 ns ns
tgtgcaatg acctctaaaacaa taatctttgggcaa 1/31(3%)
Remaining 22 of 31 sequences are unique when compared to each other and are shown in the supporting information section, S5 Table.