Skip to main content
. 2019 Jul 16;14(7):e0219664. doi: 10.1371/journal.pone.0219664

Fig 3. Cleavage of the proposed disulfide bond, 44C/254C, by DTT in hINMT.

Fig 3

Left side: hINMT crystal structure prepared without reducing agent with the position of common miss-sense SNPs (D28N, M206V, G219E and C254 [note: C44 is not at a position of a common SNP]) red-highlighted. Right side: Reducing agent-induced cleavage of proposed disulfide bond causes an increase in 254C enzyme activity; N-terminal tandem alpha-helical regions blue-highlighted; N- and C-termini, green and blue highlighted respectively.