Figure 3.

WT astrocytes rescue the dendritic morphology of Fmr1 KO neurons. Fmr1 KO E17 primary hippocampal neurons were cultured for 7 DIV with primary cortical astrocytes from either WT or Fmr1 KO mice. a, Neurons stained with an antibody directed against the neuronal dendritic marker, MAP2. The left and right images are representative of Fmr1 KO neurons cultured on WT or Fmr1 KO astrocytes, respectively. Scale bar, 50 μm. b, Representative dendritic arbor skeletons of Fmr1 KO neurons grown on WT astrocytes (left panel) or Fmr1 KO astrocytes (right panel). c–h, Quantification of dendritic arbor morphology. Data shown are mean values ± SEM. WT: n = 318, N = 3; Fmr1 KO: n = 201, N = 2. Significant differences revealed by Mann–Whitney U tests (two-tailed) are indicated (***p < 0.001). The horizontal dotted line indicates mean value for WT neurons grown on WT astrocytes (from Fig. 2c–h).