The effect of Eph–Fc in solution on Schwann cell migration on astrocytes. A–B′, Immunostaining for EphA4 (A) and EphA2 (B) in postnatal Schwann cells and their corresponding negative controls (A′, B′). DAPI nuclear staining is blue. Scale bar, 75 μm. C, D, EphA4–Fc improves Schwann cell migration on astrocytes near the coverslip in the Schwann cell migration assay, but EphA2–Fc did not have any effect. n = 3; t test, *p < 0.05.