Figure 5.

Inclusion of neurospecific E8a in LSD1 transcripts is dynamically modulated during neuronal development, whereas inclusion of E2a is a steady event. The relative amount of each LSD1 isoform was measured by rqf-PCR; cDNA were obtained from total RNA from rat embryonic cortex (E18.5), postnatal rat cortex (PN), and adult rat cortex. Graphs represent the relative percentage of each isoform with respect to the sum of the four. Only two isoforms are shown per graph; A compares LSD1 isoform with LSD1-8a, whereas B compares LSD1-2a isoform with LSD1-2a/8a. Values shown are mean ± SD. C, Exon inclusion frequency of exons E2a and E8a. Each represented series relates to the overall inclusion of either E8a (black squares) calculated as the sum of LSD1-2a/8a and LSD1-8a relative percentage and E2a (white squares), calculated as sum of LSD1-2a and LSD1-2a/8a relative percentage, at each indicated developmental stage. D, Total LSD1 transcript quantification by qRT-PCR on total RNA extract from the indicated rat cortex samples normalized on β-actin. Samples are expressed as fold increase relative to the LSD1 value at E18.5. Western blot on total protein samples from the indicated development cortical stages with a panLSD1 antibody or neurospecific LSD1 antibody (E) and with the indicated synaptic markers (F).