Figure 8.

Effect of overexpression of neurospecific or ubiquitous LSD1 isoforms on neurite morphology in rat cortical neurons. Primary rat cortical neurons were transiently cotransfected with pCGN vector (mock) (A), EGFP together with HA–LSD1-8a plus LSD1-2a/8a (nLSD1) (B), HA–LSD1 plus LSD1-2a (uLSD1) (C). Morphology was analyzed in EGFP-positive neurons (mock) or double-labeled EGFP- and HA-positive neurons. D, Cumulative neurite length in differentially LSD1 transfected neurons is indicated as average ± SEM in micrometers. E, Secondary branches count is indicated ± SEM. F, Average neurite width by Sholl analysis calculated on inner, intermediate, and outer Sholl's circles corresponding to 20, 25, and 30 μm radii, respectively. Values shown are mean ± SEM width in micrometers. Student's t test (l Stat t I ≥ T α) was applied to values by comparing each condition with mock. *p < 0.05; **p < 0.01; ***p < 0.001. Scale bars, 20 μm.