Figure 4.

Localization of oxidative stress and PV-IR in DG and CA3 of ventral hippocampus of adult GCLM +/+ and −/− mice. A, E, DAPI staining of cell nuclei (blue). B, F, DNA oxidation as revealed with 8-Oxo-dG labeling (green). C, G, PV-IR (red). D, H, Colocalization of the markers. Note the strong 8-Oxo-dG labeling in CA3 and hilar cells annexed to the DG in GCLM −/− (F) but not in +/+ (B) mice. A reduction in density of PV-IR FSI (cell bodies) particularly in DG of GCLM −/− (G), as compared to +/+ (C) mice, is also observed. Scale bar, 200 μm.