Figure 7.

Localization of oxidative stress and PV-IR in dorsal hippocampus of adult GCLM +/+ and −/− mice. A, E, DAPI staining of cell nuclei (blue). B, F, DNA oxidation as revealed with 8-Oxo-dG labeling (green). C, G, PV-IR (red). D, H, Colocalization of the markers. Note that 8-Oxo-dG labeling is almost absent in both genotypes (B, F). This contrasts with the strong 8-Oxo-dG labeling observed in ventral hippocampus of adult GCLM −/− mice (Fig. 4F). The absence of 8-Oxo-dG labeling in dorsal hippocampus of both genotypes was observed systematically in all 5 GCLM −/− and 5 +/+ mice investigated. Scale bar, 200 μm.