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. 2010 Feb 17;30(7):2433–2441. doi: 10.1523/JNEUROSCI.5880-09.2010

Figure 1.

Figure 1.

5-HT1AR exerts negative control on nNOS expression. A, B, Immunoblots of nNOS in the cultured hippocampal neurons incubated with 5-HT or 8-OH-DPAT for 24 h at the concentrations indicated. C, D, Immunoblots of nNOS in the cultured hippocampal neurons incubated with 10−5 m 5-HT for 24 h in the presence of NAN-190 at the concentrations indicated. E, F, Immunoblots of nNOS in the hippocampus of mice treated with 8-OH-DPAT or NAN-190 for 1, 3, or 7 d. G, Reverse transcription PCR examining mRNA levels of nNOS from the hippocampus of mice treated with 8-OH-DPAT or vehicle for 1, 3, and 7 d. H, Representative of nNOS immunofluorescence from the cultured hippocampal neurons incubated with 10−5 m 8-OH-DPAT or vehicle for 24 h. I, Immunoblots of nNOS in the hippocampus of KO or WT mice exposed to hippocampal 10−5 m 8-OH-DPAT microinjections for 24 h. Scale bar, 20 μm. In G–I, similar results were observed in each of four experiments. Error bars represent means ± SEM. n = 3–4; *p < 0.05 versus control. DPAT, 8-OH-DPAT.