Figure 3.

Deletion of Munc13-3 enhances the paired-pulse facilitation at GC → BC synapse, but GC → PC and GC → SC synapses are unaltered. A, An example trace of EPSCs recorded from a basket cell of Munc13-3 KO mice (top) shows no depression. The cell was voltage clamped at −65 mV in the presence of SR 95531, and 50 Hz trains of stimulation were applied to the granule cell layer. Normalized EPSCs were averaged from six recordings in KO mice (open circles in bottom) present a significant increase of the paired-pulse ratio compared with their wild-type littermates (filled circles). *p < 0.01. B, The same as A, but the postsynaptic cells were Purkinje cells and stellate cells.