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. 2010 Jun 16;30(24):8138–8150. doi: 10.1523/JNEUROSCI.0256-10.2010

Figure 4.

Figure 4.

dCIP4 controls postsynaptic Wsp localization. A1–A4, A single confocal slice of an NMJ 6/7 triple stained with anti-dCIP4 (green), anti-Wsp (red), and anti-Myc (blue) is shown for wild-type (WT; w1118) larvae overexpressing Myc-tagged Cdc42V12 in muscles. Insets show higher-magnification images of single Ib boutons labeled with asterisks. B1–C2, Single confocal slices of NMJs 6/7 double stained with anti-dCIP4 (red) and anti-HRP (green) are shown for wild-type (yw; B1, B2) and cdc422/cdc422 mutant (C1, C2) larvae. D1–E2, Single confocal slices of NMJs 6/7 double stained with anti-Wsp (red) and anti-HRP (green) are shown for wild-type (w1118; D1, D2) and dcip41/Df(3L)ED4342 mutant (E1, E2) larvae. F1–I3, Single confocal slices of NMJs 6/7 triple stained with anti-HA (green), anti-Wsp (red), and anti-HRP (blue) are shown for dcip41/Df(3L)ED4342 mutant larvae postsynaptically expressing wild-type HA-dCIP4 (F1–F3), HA-dCIP4–L145D [dCIP4(LD); G1–G3], HA-dCIP4–L436S [dCIP4(LS); H1–H3], HA-dCIP4–W603K [dCIP4(WK); I1–I3]. Postsynaptic expression of wild-type dCIP4, dCIP4–L145D, or dCIP4–L436S restores normal postsynaptic localization of endogenous Wsp in dcip41/Df(3L)ED4342 mutant larvae, whereas dCIP4–W603K fails to do so. Scale bar, 10 μm.