Figure 8.

Double-labeling immunofluorescence of COX-2 with GFAP⁺ astrocytes, or Iba1⁺ microglial cells, or NeuN⁺ neurons in the medial frontal cortex of the WT and TLR4-KO mice treated with or without ethanol for 5 months. Images were acquired using a Leica TCS-SP2-AOBA confocal laser-scanning microscope. Immunolabeling of COX-2 (red) with GFAP⁺ astrocytes (A), or with Iba1⁺cells (B), or with NeuN⁺ neurons (C) is shown. A high proportion of the immunoreactivity of COX-2 colocated with astrocytes or microglia or neurons from the brain sections from ethanol-treated WT mice (asterisks). Scale bars, 75 μm. Higher-magnification images from boxes, 15 μm.