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. 2010 Jun 23;30(25):8581–8590. doi: 10.1523/JNEUROSCI.5486-09.2010

Figure 1.

Figure 1.

Absence of LH release after peripheral NMDA injection in Kiss1- and Gpr54-null mice. A, Bar graph showing the mean ± SEM of LH levels in wild-type (+/+), Kiss1-null (Kiss−/−), and Gpr54-null (Gpr54−/−) adult male mice 10 min after PBS, NMDA, or Kp10 intraperitoneal injection. B, Bar graph showing the mean ± SEM of plasma LH levels in prepubertal mice of each genotype 10 min after PBS, NMDA, or Kp10 intraperitoneal injection. **p < 0.01 (one-way ANOVA, followed by Student–Newman–Keuls test). Numbers in brackets indicate animal number used in each treatment group. C, Representative coronal sections of dual-labeled immunocytochemistry showing the absence of c-Fos staining (black nuclei) in GnRH neurons (brown) in hypothalamus from wild-type or Kiss1-null mice that showed high plasma LH after peripheral stimulation with NMDA (left) or Kp10 (middle and right). Scale bar, 100 μm. All photographs are the same scale. Inset box is 4× magnification. 3V, Third ventricle.