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. 2010 Aug 4;30(31):10360–10368. doi: 10.1523/JNEUROSCI.1041-10.2010

Figure 3.

Figure 3.

Peripheral and spinal T-channels are not involved in the development of acid-induced chronic hyperalgesia. A, Intramuscular injection of ETX (0.1 and 0.2 mg) or NNC 55-0396 (NNC, 1 μg) did not prevent acid-induced chronic hyperalgesia. B, Current–voltage curves of T-currents recorded from Fluorogold-labeled muscle afferents DRG neurons. Numbers in parentheses indicate the number of cells recorded. Inset shows representative current traces evoked by a depolarizing step to −40 mV from a holding potential of −90 mV. C, Intrathecal injection of ETX (0.1 and 0.2 mg) or NNC (1 μg) did not prevent acid-induced chronic hyperalgesia. D, The induction of spinal c-Fos expression in response to repeated acid injection was similar in WT and Cav3.2−/− mice. E, The number of c-Fos-positive cells in the dorsal horn in WT and Cav3.2−/− mice that received pH 4.0 or pH 7.2 saline. Averaged numbers of c-Fos-positive cells from three consecutive sections were counted per animal. Three animals were used for each treatment. *p < 0.05, compared with group treated with pH 7.2 saline. Error bars represent SEM.