Figure 6.

Screening for extrinsic and non-Kenyon cell intrinsic neurons of the mushroom body. For each of the seven analyzed types of MBENs and two types of non-KC MBINs, the expression pattern (anti-GFP channel; A–I), the innervation of the MB neuropil (anti-GFP and anti-Fasciclin II/anti-Choline acetyltransferase channels [magenta: neuropil marker]; A′–I′), its overlap with a 3D reconstruction of the MBs (A″–I″) and its categorization according to the innervated MB subdomain (right) is shown. MBENs innervate the tip (V3 subdomain; A), shaft (V2; B), or base of the vertical lobe (V1; C), the spur (D), the lateral appendix (LA; E), the medial appendix (MA; F), or the calyx (G). MBENs specifically innervate the pedunculus (H) or the medial lobe (M1 subdomain; I). vl, Vertical lobe of the mb; sp, spur; la, lateral appendix; ml, medial lobe of the mb; ma, medial appendix; al, antennal lobe; ca, calyx; act, antennocerebral tract; ped, pedunculus.