Figure 3.
Microinjections of antibodies against synaptophysin or dynamin increase duration and quantal size of depolarization-induced exocytotic events. Chromaffin cells were injected with anti-Syn, anti-Dyn or anti-His. The exocytosis response was evoked by high K+ and measured 30–45 min after injections. A, Representative amperometric traces from noninjected cells (Control) or cells injected with anti-Syn. B, Cumulative histograms of the number of amperometric events from control (white circles), anti-Syn (black squares) and anti-Dyn injected cells (gray triangles). C, Representative amperometric spikes from noninjected cells (Control), anti-Syn and anti-Dyn injected cells. D, Representative amperometric spike with its parameters: Q and t1/2. E, Averaged values for Q and t1/2. Data are means ± SEM of averages per cell (14–24 cells from 3 to 5 different cultures). *p < 0.05 compared with control cells.