Table 1.
Amperometric spike parameters from chromaffin cells in different experimental conditions
| Imax (pA) | Rise time (ms) | Decay time (ms) | |
|---|---|---|---|
| Control | 83 ± 4 | 5.2 ± 0.3 | 7.7 ± 0.5 |
| Anti-Syn (10 μm) | 81 ± 7 | 5.9 ± 0.5 | 10.6 ± 0.8* |
| Anti-Dyn (10 μm) | 78 ± 6 | 5.4 ± 0.4 | 10.3 ± 0.7* |
| Anti-His (10 μm) | 77 ± 4 | 5.5 ± 0.4 | 8.1 ± 0.4 |
| GST (40 μm) | 88 ± 8 | 4.8 ± 0.3 | 7.3 ± 0.5 |
| GST-Cterm (10 μm) | 95 ± 4* | 4.9 ± 0.4 | 8.6 ± 0.6 |
| GST-Cterm + anti-Syn (10 μm) | 89 ± 5 | 4.7 ± 0.2 | 7.2 ± 0.4 |
| GST-Cterm (40 μm) | 89 ± 4 | 4.8 ± 0.4 | 11.9 ± 0.6* |
| GST-Cterm + anti-Syn (40 μm) | 90 ± 9 | 4.5 ± 0.5 | 7.1 ± 0.6 |
| C-term240-290 (10 μm) | 92 ± 4 | 5.7 ± 0.5 | 10.6 ± 1.0* |
| C-term240-290 + anti-Syn (10 μm) | 85 ± 9 | 5.0 ± 0.3 | 7.1 ± 0.3 |
Control cells correspond to noninjected cells. Cells were injected with monoclonal anti-Syn, anti-Dyn, or anti-His antibodies. Cells were also injected with the following peptides: GST, GST-Cterm, and C-term240-290. Peptides encompassing the C terminal of synaptophysin were also coinjected with anti-Syn (GST-Cterm + anti-Syn; C-term240-290 + anti-Syn). Rise time, Time needed to reach Imax; Decay time, time constant of a single exponential fit to the decay phase. Data are means ± SEM of cell median for each spike parameters (12–24 cells from 3–5 different cultures).
*p < 0.05 compared with control cells.