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. 2010 Oct 6;30(40):13441–13453. doi: 10.1523/JNEUROSCI.0907-10.2010

Figure 3.

Figure 3.

A survey of the subcellular localization of SV-enriched Rabs in rat hippocampal neurons. pEGFP-C2-Rab3a, -Rab4b, -Rab5a, -Rab10, -Rab11b, -Rab14, and -Rab27b were each transiently transfected into primary rat hippocampal neurons (10–12 DIV). Forty-eight hours after transfection, neurons were fixed and immunostained with antibodies against Syph (red) and MAP2 (blue) to visualize synaptic boutons and dendrites, respectively. Areas of overlap appear yellow. Arrows highlight colabeled structures. Scale bar, 20 μm.