Table 1.
Complete survey of the rat SV Rabome
| Rab isoform | Gi number | Quantified by | WB ratio (±SD) |
iTRAQ ratio (±SD) |
Localization by |
GDI Pool IP/MS detection | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S3/H | LP2/H | SV/H | S3/H | LP2/H | SV/H | EGFP | AB | SB labeling | ||||
| Rab1a | gi|45433570 | MS & WB | 0.47 (0.33) | 2.86 (0.52) | 3.60 (0.61) | 0.12 (0.29) | 2.87 (0.19) | 5.29 (0.63) | Yes | Yes | No | Yes |
| Rab2a | gi|13929006 | MS & WB | 0.34 (0.08) | 2.93 (0.04) | 3.79 (0.41) | 0.39 (0.05) | 2.81 (0.22) | 4.93 (0.96) | N/A | Yes | No | Yes |
| Rab2b | gi|83415090 | MS | 0.34 (0.08) | 2.93 (0.04) | 3.79 (0.41) | 0.36 (N/A) | 1.04 (N/A) | 1.06 (N/A) | N/A | Yes | No | Yes |
| Rab3a | gi|61098195 | MS & WB | 2.43 (0.32) | 7.66 (0.16) | 12.42 (0.72) | 0.38 (0.04) | 6.11 (1.52) | 13.07 (1.40) | Yes | Yes | Strong | Yes |
| Rab3b | gi|13592037 | MS & WB | 1.05 (0.28) | 4.98 (0.32) | 10.53 (0.73) | 0.41 (0.01) | 4.23 (1.38) | 6.62 (0.56) | N/A | Yes | Strong | Yes |
| Rab3c | gi|19424194 | MS & WB | 0.45 (0.02) | 6.30 (0.82) | 9.89 (1.85) | 0.30 (0.01) | 6.80 (0.31) | 19.04 (1.84) | Yes | N/A | Strong | Yes |
| Rab4a | gi|77404180 | WB | 1.30 (0.03) | 1.80 (0.89) | 5.01 (0.24) | N/A | N/A | N/A | N/A | N/A | N/A | Yes |
| Rab4b* | gi|8394136 | MS & WB | 1.30 (0.03) | 1.80 (0.89) | 5.01 (0.24) | 0.05 (N/A) | 0.90 (N/A) | 1.84 (N/A) | Yes | N/A | Weak | Yes |
| Rab5a | gi|12083645 | MS & WB | 1.24 (0.03) | 2.63 (0.60) | 6.91 (0.27) | 0.04 (0.25) | 2.18 (0.19) | 5.30 (1.35) | Yes | Yes | Moderate | Yes |
| Rab5b | gi|121583768 | MS & WB | 1.24 (0.53) | 2.63 (0.60) | 6.91 (0.27) | 0.35 (0.18) | 2.79 (0.70) | 5.68 (0.20) | N/A | Yes | Moderate | Yes |
| Rab5c | gi|157786692 | MS & WB | 1.24 (0.53) | 2.63 (0.60) | 6.91 (0.27) | N/A | 1.51 (N/A) | 3.30 (N/A) | N/A | Yes | Moderate | Yes |
| Rab6a | gi|213972608 | MS & WB | 1.54 (0.36) | 1.35 (0.48) | 1.68 (0.42) | 0.73 (N/A) | 2.58 (N/A) | 2.59 (N/A) | N/A | Yes | No | Yes |
| Rab6b | gi|157817539 | MS & WB | 1.54 (0.36) | 1.35 (0.48) | 1.68 (0.42) | 0.54 (N/A) | 2.28 (N/A) | 3.37 (N/A) | Yes | Yes | No | Yes |
| Rab7a | gi|13027392 | MS & WB | 1.06 (0.87) | 1.11 (0.27) | 4.47 (0.67) | 2.29 (0.05) | 2.13 (0.24) | 3.71 (0.16) | Yes | N/A | No | Yes |
| Rab8b | gi|23463313 | MS | N/A | N/A | N/A | N/A | 1.59 (N/A) | 1.43 (N/A) | Yes | N/A | No | Yes |
| Rab10 | gi|61889071 | MS & WB | 0.34 (0.44) | 3.36 (0.62) | 6.30 (0.3) | 0.28 (0.01) | 2.81 (0.38) | 6.33 (0.09) | Yes | N/A | Moderate | Yes |
| Rab11b | gi|14249144 | MS & WB | 0.76 (0.07) | 3.28 (0.02) | 4.67 (0.20) | 0.46 (0.22) | 3.01 (0.15) | 4.28 (0.63) | Yes | N/A | Moderate | Yes |
| Rab12* | gi|158186685 | — | N/A | N/A | N/A | N/A | N/A | N/A | Yes | N/A | No | Yes |
| Rab14 | gi|16758368 | MS & WB | 0.74 (0.17) | 3.74 (0.08) | 6.94 (0.42) | 0.52 (0.02) | 3.99 (0.58) | 9.95 (0.75) | Yes | N/A | Moderate | Yes |
| Rab15 | gi|38454238 | MS | N/A | N/A | N/A | N/A | 2.79 (0.32) | 3.76 (0.26) | N/A | N/A | N/A | No |
| Rab18 | gi|60223069 | MS | N/A | N/A | N/A | 0.12 (0.38) | 2.94 (0.17) | 2.05 (0.06) | Yes | N/A | No | Yes |
| Rab21 | gi|51948448 | MS | N/A | N/A | N/A | N/A | 3.22 (0.94) | 2.64 (0.55) | N/A | N/A | N/A | Yes |
| Rab26* | gi|19424272 | MS | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | Yes |
| Rab27b | gi|16758202 | WB | 0.22 (0.29) | 4.03 (0.65) | 9.95 (0.31) | N/A | N/A | N/A | Yes | Yes | Strong | No |
| Rab33b | gi|157822117 | MS & WB | 1.33 (0.57) | 2.10 (0.44) | 3.93 (1.06) | 0.36 (N/A) | 1.04 (N/A) | 1.06 (N/A) | Yes | N/A | No | Yes |
| Rab34/39# | gi|58865850/gi|157822741 | MS & WB | 0.44 | 2.45 (0.81) | 3.98 (0.63) | N/A | N/A | N/A | N/A | N/A | N/A | No |
| Rab35 | gi|61556789 | MS & WB | 0.17 (0.06) | 2.61 (0.34) | 4.34 (0.08) | 0.63 (N/A) | 1.85 (N/A) | 1.96 (N/A) | Yes | Yes | No | Yes |
| Rab43 | gi|66730539 | — | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | N/A | No |
*Peptide redundancy. Rab4b: 1a, 3a–c, 4a, 4b, 8b, 10, 12; Rab12: 1a, 3a–b, 4a, 8a, 10, 14, 35; Rab26: 1a, 3a–b, 4a, 8a, 10, 14, 35.
#Unique peptide poor quality for quantitation. Rab34/39.
Note that localization studies were performed using EGFP-fused Rabs and/or Rab-specific antibodies (AB). SB labeling was scored weak-to-strong based on Rab colocalization and correlation with synaptophysin immunostaining. N/A, Not applicable; WB, Western blotting.