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Composition of intracellular solutions
| Solution | BAPTA | EGTA | CaCl2 | Free [Ca2+]i | MgCl2 | MgATP | NaGTP | HEPES | CsCl | KCl |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 20 | 0.1 | 1 | 1 | 4 | 0.4 | 10 | 134 | ||
| 2 | 20 | 6.4 | 112 | 1 | 4 | 0.4 | 10 | 124 | ||
| 3 | 2 | 0.01 | 1 | 1 | 4 | 0.4 | 10 | 142 | ||
| 4 | 10 | 0.1 | 1 | 1 | 4 | 0.4 | 10 | 132 | ||
| 5 | 10 | 4.8 | 112 | 1 | 4 | 0.4 | 10 | 125 | ||
| 6 | 10 | 1 | 80 | 1 | 4 | 0.4 | 10 | 134 |
Concentrations are expressed in millimolar except for free [Ca2+]i (nm). pH adjusted to 7.3 with CsOH (Solutions 1–5) or KOH (Solution 6); 297–303 mOsm/L.
