Figure 2.

Rescue of the juxtaparanodal complex by glial TAG-1 expression in the optic nerve. A–F, Immunohistochemistry of longitudinal optic nerve cryosections for Caspr and the juxtaparanodal proteins Caspr2 and Kv1.2. Caspr2 and Kv1.2 are normally clustered in juxtaparanodes of Tag-1^+/− (A, B) and Tag-1^−/−;plp^Tg(rTag-1) (E, F; asterisks denote juxtaparanodal staining) mice, whereas their localization is disrupted in the Tag1^−/− optic nerve (C, D). G, H, Quantification of the percentage of paranodes stained with Caspr having normally clustered Caspr2 (G) or Kv1.2 (H) in Tag-1^+/−, Tag-1^−/−, and Tag-1^−/−;plp^Tg(rTag-1) mice (G and H, mean percentages, 70.60 and 64.09 for Tag-1^+/−; 9.38 and 25.32 for Tag-1^−/−; and 70.20 and 62.81 for Tag-1^−/−;plp^Tg(rTag-1), respectively). Student's t test, p < 0.05 (G); p < 0.05 (H) between Tag-1^−/− and Tag-1^−/−;plp^Tg(rTag-1), no statistically significant difference was detected between Tag-1^−/−;plp^Tg(rTag-1) and normal littermates, n = 3 of each genotype. Bar graphs depict mean ± SEM. ***p < 0.05. JXP, Juxtaparanodes. Scale bar, 10 μm.