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. 2019 Jul 16;14(7):e0218989. doi: 10.1371/journal.pone.0218989

Fig 6. Molecular markers with significant differential expression and prognostic efficacy in laryngopharyngeal cancers with angiolymphatic invasion and nodal metastasis.

Fig 6

Validation of the differentials identified in the laryngopharyngeal cancer within the TCGA cohort of patients with angiolymphatic invasion identified 4 genes with significant difference in mRNA expression levels (z-score) in TCGA cohort laryngopharyngeal cancer with ALI; the top three genes being MFN1, PAK2 and FXR1 (A-C). Among this cohort, SLC4A1AP showed an association in both OS (D; p = 0.002785) and DFS (E; p = 0.0287) of ALI+ patients, while PAK2 showed association with ALI- in DFS alone (F; p = 0.033). Prognostic assessment of the subset of meta-analysis based differentials altered in >20% of the patients identified the combination of RYK and IL1RAP to be associated with poor survival (G and H). EPS8 was the only differential in node in node+ECS- patients with no survival impact (I) validation of the differentials associated with node positive with/without ECS indicated that EPS8 was the only differential (downregulated) in node+/ECS+ cohort with no association with survival. Prognostic efficacy (subset altered in >20% patients) indicated that STK3, TMEM267 and PSMD2 were the prognosticators of poor survival (J and K). (P-value; * p<0.05; ** p<0.005).