Figure 3.

YAC128 mice show a progressive striatal-specific decrease in pS421-htt, but normal levels of htt phosphorylation are observed in YAC18 and C6R mice. A, B, Brain tissue was isolated at the indicated time points and the level of pS421-htt was determined in striatum (A) and cerebellum (B) after immunoprecipitation of htt, Western blot, and probing with Abs against pS421-htt and total htt (mAb 2166) followed by probing with secondary Abs. Quantification of immunoreactivity was performed with a LI-COR system using Odyssey software. The ratio between pS421-htt and total htt was determined and expressed relative to WT for each tissue, time point, and genotype in three independent experiments. Relative levels of phosphorylation for mutant htt (mhtt) and endogenous htt (ehtt) were pooled. *p < 0.05 compared with WT, by t test. Error bars denote ±SEM.