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ATF4 regulates Puma induction during ER stress but not DNA damage. Cortical neurons derived from ATF4−/− and ATF4+/+ littermates were treated with the ER stressors tunicamycin (3 μg/ml; TM) and thapsigargin (1 μm; TG) or the DNA-damaging agents camptothecin (10 μm; CPT) and etoposide (10 μm; ETP), and RNA was extracted after 8 and 12 h. Puma mRNA levels were quantified by real-time RT-PCR and are reported as fold increase over corresponding untreated controls (n ≥ 5; *p < 0.01).
