Skip to main content
. 2010 Dec 15;30(50):16938–16948. doi: 10.1523/JNEUROSCI.1598-10.2010

Figure 9.

Figure 9.

ATF4 regulates CHOP induction in response to ER stress but not DNA damage. A, Cortical neurons were treated with camptothecin (10 μm; CPT), tunicamycin (3 μg/ml; TM), or thapsigargin (1 μm; TG), and CHOP mRNA levels were quantified by real-time RT-PCR after 2, 4, and 8 h (n = 4; *p < 0.01). Ctrl, Control. B, CHOP protein levels were assessed by Western blot 10 h after treatment with camptothecin, tunicamycin, or thapsigargin. NT, Nontreated. C, Cortical neurons derived from ATF4+/+ and ATF4−/− littermates were treated with camptothecin, tunicamycin, or thapsigargin, and, after 8 h, CHOP mRNA levels were quantified by real-time RT-PCR (n ≥ 4; *p < 0.01). D, ATF4+/+ and ATF4−/− cortical neurons were treated with tunicamycin and after 8 and 12 h CHOP protein levels were assessed by Western blot.