Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identi-fiers | Additional information |
|---|---|---|---|---|
| Cell line (Human) | U2OS cdk1-as | Rata et al., 2018 | ||
| Cell line (Human) | wild-type U2OS | ATCC | HTB-96 | |
| Cell line (Human) | HeLa | ATCC | CCL-2 | |
| Cell line (Human) | hTERT RPE | ATCC | CRL-4000 | |
| Transfected construct (Human) | p3'SSclonedimer pF9-poly-EGFP-lacI-NLS (GFP-LacI-NLS) | Hori et al., 2013 | ||
| Transfected construct (Human) | mCherry-Lifeact-7 (mCherry-Lifeact) | Addgene | #54491 | A gift from Michael Davidson |
| Transfected construct (Human) | pGFP-alphaTub (GFP-Tubulin) |
Other | A gift from Jason Swedlow lab | |
| Transfected construct (Human) | mRFP1-SR-KASH (LINC-DN) | Luxton et al., 2010 | ||
| Transfected construct (Human) | mRFP1-KASH-ΔL (Control) | Luxton et al., 2010 | ||
| Transfected construct (Human) | pCS-H2B-cerulean (H2B-Cerulean) | Other | A gift from Sean Megason Lab | |
| Transfected construct (Human) | CMV-GFP-NMHC II-A (MHC-GFP) | AddGene | #11347 | A gift from Robert Adelstein |
| Antibody | anti-Tubulin (Rat monoclonal) | Cell Signalling Technology | YL1/2 | Used at 1:1000 dilution |
| Antibody | anti-LaminB1 (Rabbit polyclonal) | Abcam | ab16048 | Used at 1:1000 dilution |
| Antibody | anti-Actin (Rabbit polyclonal) | Thermo-Fisher | PA1-183 | Used at 1:1000 dilution |
| Antibody | A488 goat anti-Rabbit | Thermo-Fisher | A-11034 | Used at 1:1000 dilution |
| Antibody | A488 donkey anti-Rat | Thermo-Fisher | A-21208 | Used at 1:1000 dilution |
| Antibody | A594 goat anti-Rabbit | Thermo-Fisher | A-11037 | Used at 1:1000 dilution |
| Chemical compound, drug | Phalloidin DyLight 650 | Invitrogen | 13454309 | Used at 1:1000 dilution |
| Chemical compound, drug | Hoechst 33342 | Sigma-Aldrich | 14533 | Used at 1:2000 dilution |
| Chemical compound, drug | SiR-DNA | tebu-bio | SC007 | Used at 100 nm |
| Chemical compound, drug | 1NM-PP1 | Merck Millipore | 529581 | Used at 1 μM |
| Chemical compound, drug | RO-3306 | Merck Millipore | 217699 | Used at 10 μM |
| Chemical compound, drug | nocodazole | Sigma | M1404 | Used at 3.3 μM |
| Chemical compound, drug | paranitroblebbistatin (pnBB) | motorpharmacology | Used at 50 μM | |
| Chemical compound, drug | azidoblebbistatin (azBB) | motorpharmacology | Used at 5 μM | |
| Chemical compound, drug | jasplakinolide | Merck | J4580 | Used at 1 μM |
| Chemical compound, drug | MK-1775 | Selleck Chemicals | S1525 | Used at 500 nM |
| Software, algorithm | Imaris, 3D surface tool | Bitplane | ||
| Software, algorithm | Imaris, Convex Hull generation tool | Bitplane | Convex Hull tool from filament tool adapted for 3D surface tool |
|
| Software, algorithm | Fiji | https://fiji.sc/ |