Author response image 8. DRG neurons were harvested from 16-17 mo GFP-LC3B transgenic mice and co-transfected with the indicated mCherry-mAtg8 construct and Halo-ATG5 to mimic the imaging experiments.

Neurons were grown for 2 DIV, harvested, and immunoblotted for mCherry. Total protein for each lane was quantified: 100, 47, 83, and 57 for each lane, from left to right.