Video 1. Productive biogenesis events in a neuron from an aged mouse.

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DOI: 10.7554/eLife.44219.006

GFP-LC3B and mCh-ATG13 in the distal neurite of a DRG neuron from an aged mouse depicting a productive autophagosome biogenesis event. In the merge movie (bottom), yellow arrowheads denote colocalization of ATG13 and LC3B; green arrowheads denote a LC3B-positive punctum from which ATG13 has dissociated; solid arrowheads track one punctum, hollow arrowheads follow a second punctum. Retrograde is to the right. Scale bar, 2 μm. Playback at five frames per second. Movie stills are shown in Figure 3A.