Table 2:

Molecular Tumor Infiltrating Lymphocytes and Immune Subset Signatures Stratified by Age ≤ 40 Versus > 40 Years Old in NCCTG N9831

mTIL Signatures	Age ≤ 40 (n=281)	Age > 40 (n=1,111)	Total (N=1,392)	Mann-Whitney P Value
CD45				.004
Mean	−0.2	0.0	0.0
Median (range)	−0.2 (−2.4-2.4)	0.0 (−2.8-2.9)	−0.1 (−2.8-2.9)
B cells				.09
Mean	−0.2	0.0	0.0
Median (range)	−0.3 (−2.7-3.4)	−0.1 (−3.0-4.1)	−0.2 (−3.0-4.1)
CD8 T cells				.14
Mean	−0.1	0.0	0.0
Median (range)	−0.1 (−2.8-2.3)	0.0 (−3.0-2.5)	0.0 (−3.0-2.5)
Cytotoxic cells				.03
Mean	−0.2	0.0	0.0
Median (range)	−0.2 (−3.0-2.8)	0.1 (−2.9-3.6)	0.0 (−3.0-3.6)
Exhausted CD8				.02
Mean	−0.1	0.0	0.0
Median (range)	−0.1 (−1.3-1.4)	0.0 (−1.4-2.2)	0.0 (−1.4-2.2)
iDC				.17
Mean	−0.1	0.0	0.0
Median (range)	−0.1 (−3.2-2.0)	0.1 (−2.6-2.6)	0.0 (−3.2-2.6)
Macrophages				.0003
Mean	−0.2	0.0	0.0
Median (range)	−0.2 (−2.2-1.8)	0.0 (−3.4-3.0)	0.0 (−3.4-3.0)
Mast cells				.08
Mean	0.1	0.0	0.0
Median (range)	0.2 (−5.3-4.5)	0.1 (−5.4-4.5)	0.1 (−5.4-4.5)
Neutrophils				.0005
Mean	−0.1	0.0	0.0
Median (range)	−0.1 (−2.9-1.7)	0.0 (−2.7-2.9)	0.0 (−2.9-2.9)
NK-CD56dim cells				.001
Mean	−0.2	0.0	0.0
Median (range)	−0.2 (−3.2-2.1)	0.0, (−2.9-2.9)	0.0 (−3.2-2.9)
T cells				.02
Mean	−0.1	0.0	0.0
Median (range)	−0.2 (−2.6-2.4)	0.0 (−2.9-2.5)	0.0 (−2.9-2.5)
Treg				.04
Mean	−0.1	0.0	0.0
Median (range)	−0.1 (−2.0-1.6)	0.0 (−2.4-2.0)	0.0 (−2.4-2.0)

Abbreviations: iDC, immature dendritic cell ; mTIL, molecular tumor infiltrating lymphocyte; NCCTG, North Central Cancer Treatment Group; NK, natural killer; Treg, regulatory T cells