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. Author manuscript; available in PMC: 2019 Jul 16.
Published in final edited form as: Free Radic Biol Med. 2018 Nov 27;131:50–58. doi: 10.1016/j.freeradbiomed.2018.11.032

Fig. 1.

Fig. 1

WhiB3 protects Mtb from Oxidative and Nitrosative stress. Exponentially grown cells of Mtb, MtbΔwhiB3 and whiB3 Comp strains expressing Mrx1-roGFP2 were exposed to (A) 10 μM, 50 μM and 100 μM CHP, (B) 1 mM H2O2, (C) 5 mM H2O2, (D) 0.5 mM acidified (pH 5.5) NaNO2 and (E) 50 μM sodium peroxynitrite (OONO-) and survival was monitored by enumerating CFUs at indicated time intervals. (F) Naïve RAW264.7 macrophages were infected with Mtb, MtbΔwhiB3 and whiB3 Comp and intramacrophage survival was monitored at indicated time points. Error bars represent SD from the mean. * p < 0.05 (as compared to Mtb), + (p < 0.05 as compared to whiB3 Comp), and ns (no significant difference). Data are representative of at least three independent experiments.