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. 2019 Apr;11(2):181–186.

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Copyright© 2019 Iranian Neuroscience Society

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — Time course induction of Pfu protein expression. SDS PAGE analysis to gain insight on the time course analysis of Pfu DNA Polymerase expression following IPTG induction after 1, 2, 3, 4, 5 & 6 hours. Lane 1: Protein size marker, Lane 2: 0 hour (before IPTG was added), Lane 3: 1 hour, Lane 4: 2 hours, Lane 5: 3 hours, Lane 6: 4 hours, Lane 7: 5 hours, Lane 8: 6 hours. Pfu Polymerase (arrow) band with the size of 90 kDa was seen to be expressed. 5 hours was chosen as the optimal induction period.