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. 2019 Apr;11(2):181–186.

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Copyright© 2019 Iranian Neuroscience Society

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — Estimation of enzymatic activity for purified Pfu DNA Polymerase. (a) Gel electrophoresis of PCR product with Pfu Polymerase titration with the relative intensity of the amplicons. Pfu Polymerase from the stock was diluted in Pfu buffer (2×, 5×, 10×, 20×) accordingly. The PCR amplification was run in parallel with 1 μL of Pfu DNA Polymerase from Promega (3U/μL) and 1 μL of Pfu DNA Polymerase from Thermo Scientific (2.5U/μL). Lane 1: your marker, Lane 2: 2× dilution, Lane 3: 5× dilution, Lane 4: 10× dilution, Lane 5: 10× dilution, Lane 6: Negative Control, Lane 7: Promega (P) Pfu Polymerase, Lane 8: Thermo Scientific (TSF) Pfu Polymerase. (b) Plot Profile analysis of the bands.