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. 2019 Apr;11(2):181–186.

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Copyright© 2019 Iranian Neuroscience Society

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Analysis of Pfu DNA polymerase performance compared with commercial polymerases. (a) Gel electrophoresis of the PCR product to assess the functionality of the purified Pfu DNA polymerase in 1U. PCR amplification was carried out using p600 plasmid as the template and 1U of the purified Pfu DNA Polymerase, Promega Pfu DNA Polymerase and Thermo Scientific Pfu DNA Polymerase, respectively. Lane M: DNA size marker, Lane 1: 1U Purified Pfu Polymerase, Lane 2: 1U Promega Pfu Polymerase, Lane 3: 1U Thermo Scientific Pfu Polymerase. (b) 3D Surface Plot analysis on the intensity profile of the bands (axis units in pixels).