Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Jul 16;9:10290. doi: 10.1038/s41598-019-46700-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ABCB1 inhibition reduces migration and invasion in ependymoma cell lines. The ability of BXD1425EPN (a,c,d) and DKFZ-EP1 (b & Fig. S5) cells to migrate and invade was measured in wound healing (a,b) and spheroid invasion (c,d) assays respectively. The time required for 50% wound closure (t_1/2) was significantly increased when both the BXD-1425EPN (a) and DKFZ-EP1 (b) cell lines were treated with either the non-specific ABCB1 inhibitor, verapamil (20 µM) or the specific ABCB1 inhibitor, vardenafil (10 µM). (c) A 3D spheroid invasion assay was performed to assess the ability of ependymoma cells to digest and invade through extracellular matrix (Cultrex BME). The spheroids formed from BXD-1425EPN cell line formed invadopodia and demonstrated multicellular streaming, which was visibly reduced after 96 hours in the presence of either ABCB1 inhibitor. (d) There was a significant reduction in the relative spheroid outgrowth R (ratio of the area of the invasive edge [dotted line] to the area of the area of spheroid itself) when treated with either ABCB1 inhibitor, which became more pronounced at 96 hours. Scale bars represent 100 µm. ***p ≤ 0.005, ****p ≤ 0.001.