Table 1.
Genes included in customized PCR array*.
| Functional category | Genes |
|---|---|
| Vascular calcification | SP7, RUNX2, CDKN2A, TGM2, BGLAP, S100A12 |
| Extracellular matrix | CTGF, COL1A2, COL2A1, COL6A2, DDR1, VCAN, ELN, TIMP1, TIMP2, MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP14, ADAMTS7, VNN1, FBN1, HSPG2, ACAN, PPARG, HMGA1, EPHX2, HMGB1, COMP |
| Bone metabolism | SOST, BMP1, BMP2, BMP3, BMP7 |
| Physiological calcification inhibitors | CA2, ANKH, TRIM24, ADIPOQ, HMOX1, FTL, TNFRSF11B, MGP, AHSG, ENPP1, SPP1 |
| Ion channels | TRPV5, TRPV6, KCNN4, KCNN3, KCNMA1, RYR3, S100G |
| Endoplasmic reticulum stress | HSPA5, HSP90B1, DDIT3 |
| Apoptosis | CASP3, CASP8, BAX |
| Wnt signaling | AXIN2, SFRP4, FGF2 |
| NF-ĸB signaling | MYD88, NFKB1, RELA |
| TGF-β signaling | TGFB1, TGFBR1, TGFBR2, LTBP3, SMAD6 |
| Inflammation | IL10, TNF |
| NO synthases | NOS2, NOS3, PRKG1, NOS1 |
| Various | FGF23, KL, VDR, AGTR2, ANGPT2, SLC25A15, ALPL, IFNG, AGER, CRP |
| Housekeeping genes | HPRT1, PGK1, EIF2B1, PPIA, ELF1 |
*For abbreviations, see Supplemental Material.