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. 2019 Jul 16;10:3060. doi: 10.1038/s41467-019-11005-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Epithelial surface marker expression in vivo is unaltered during colonisation of S. pneumonia. Epithelial cells from nasal curette biopsies were analysed by flow cytometry for a CD54 and b CD107a. Results were expressed as median fluorescence intensity (left) and high cell surface expression (≥95% of the baseline expression, right) representing at least two volunteers per time point who were carriage negative (black) and carriage positive (grey). Comparisons between carriage negative and carriage positive, on each day of sampling, was analysed by one-way ANOVA (a—parametric, b—non-parametric). P = 0.84 CD54 MFI; P = 0.98 CD54 %; P = 0.09 CD107a MFI; P = 0.10 CD107a %. Boxplots represent the median (centre line) and interquartile range (box), minimums and maximums (whiskers)