Fig. 10.

ChIP-seq shows decreased H3K27Ac and H3K4me1 in HNF4α-P1 and its targets and enhanced binding of H3K27Ac to HNF4α P2 promoter. a Data were obtained from ChIP-seq of Human Liver samples from normal (n = 5) and AH (n = 6) livers. Antibodies agains Histone 3 Lysine 27 acetylation (H3K27Ac), Histone 3 Lysine 4 mono and trimethylation (H3K4me1 and H3K4me3) and Histone 3 Lysine 27 trimethylation (H3K27me3) were used in the immunoprecipitation. Integrated Genome Viewer was used to visualize BigWig peak data. b–d Genomic view of sequencing reads present in loci of b HNF4α targets PCK1, CYP3A4 and F7, c Non-HNF4α target ICAM-1 and d HNF4A. e–g Box plot of fold changes (IP to Input) of all peaks called around the TSS of e HNF4A isoforms P1 and P2, f HNF4A targets PCK1, CYP3A4 and F7 and g ICAM1. Significance was determined by two-tailed Student t test in e, f, and g: *P < 0.05, **P < 0.01, ***P < 0.001. For box-and-whisker plots in e, f, g: perimeters, 25th–75th percentile; midline, median