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. 2019 Jul 16;9:10260. doi: 10.1038/s41598-019-46727-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Exogenous administration of human low-density lipoprotein (LDL) in thyroid cell lines. (A) Percentage of cellular proliferation of the Nthy-ori 3.1 and CAL-62 cell lines. Both were treated for 24 h with LDL cholesterol (100 μg/mL and 200 μg/mL) compared with control cells maintained in basal conditions (5% LPDS). (B) Monolayer wound-induced migration assay. A line was scratched with a 200-µm plastic pipette tip in CAL-62 and Nthy-ori 3.1 cell lines; cultures were treated for 24 h with LDL cholesterol (100 mg/mL and 200 mg/mL). After 16 h, cells that had migrated to the wounded areas were photographed under a microscope for quantification of cell migration. Images are representative of three separate experiments. Quantitative analysis of wound-induced migration assay compared with control cells maintained in basal conditions (5% LPDS). The results are presented as mean ± SEM of eight experiments done in duplicate. The Kruskal–Wallis test was represented as a ± box plot, n = 8 separate experiments (**p < 0.01, ***p < 0.001).