Figure 6.

Acute Iron Deprivation Protects against Crescentic Glomerulonephritis and Induces De Novo Lipid Accumulation

(A) Experimental design.

(B) Amount of urinary protein at different time points during the course of NTN; n=3 or 4 rats/group.

(C) PAS staining showing representative glomerular morphology of the vehicle (left) and the DEF (right) groups.

(D) Glomerular fibrin score, percentage of glomeruli with crescents, and glomerular size in vehicle and DEF-treated rats; n = 3 or 4 rats/group.

(E) Nephritic glomeruli of vehicle- or DEF-treated rats showing oil red O⁺ glomerular and tubular cells in the DEF group.

(F) PCNA staining showing proliferating cells in nephritic glomeruli; number of nuclear PCNA(+) cells in the glomeruli (bottom); n = 3 or 4 rats/group.

(G) Experimental design for the therapeutic experiment.

(H) Amount of urinary protein levels at different time points during the course of NTN; n = 4 or 5 rats/group.

(I) PAS staining showing representative glomerular morphology of the vehicle (left) and DEF (right) groups.

(J) Glomerular fibrin score, percentage of glomeruli with crescents, and glomerular size in vehicle- and DEF-treated rats; n = 4 or 5 rats/group.

^∗p < 0.05, ^∗∗p < 0.01, and ^∗∗∗p < 0.001 by t test. Scale bars, 40 μm. Error bars represent SEM.

See also Figure S6.