Fig. 4.

The role of LBR in Xist-mediated silencing. a Allelic silencing across Chr3 after 1 day of Xist RNA induction in WT and Lbr null (KO) iXist-Chr3 cells. Mean value of difference of allelic ratio for each gene with an informative SNP was calculated from biological replicates as detailed in Supplementary Table 4. Green line on Chr3 ideogram indicates location of the Xist transgene. b Violin plot summarising data in a. P values were calculated using one-sided Wilcoxon rank-sum test. c Allelic silencing across ChrX after 1 day of Xist RNA induction in WT and Lbr null (KO) iXist-ChrX cells. Mean value of difference of allelic ratio for each gene with an informative SNP was calculated from biological replicates as detailed in Supplementary Table 4. Green line on ChrX ideogram indicates location of the Xist locus. d Violin plot summarising data in c and e. P values were calculated using one-sided Wilcoxon rank-sum test. e Allelic silencing across ChrX after 1 day of Xist RNA induction in WT and XistΔLBS iXist-ChrX cells. Mean value of difference of allelic ratio for each gene with an informative SNP was calculated from biological replicates as detailed in Supplementary Table 4