Figure 2.

Sph3_h and PelA_h are specific for α-1,4-GalNAc. MALDI-TOF MS analysis of the products released by the incubation of Sph3_h and PelA_h with (A) a fraction enriched in α-1,4-galactose 9-mers, (B) a fraction enriched in α-1,4-galactosamine 9-mers, and (C) chemically partially hydrolyzed chitin. The initial sample is represented in white, following Sph3_h treatment in gray, and PelA_h treatment in black. MALDI-TOF MS spectra of (D) a sample enriched in α-1,4-GalNAc 4-mer to 15-mer. MALDI-TOF MS spectra of the enzymatic products released by (E) Sph3_h and (F) PelA_h from the sample presented in D.