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. 2019 Jun 5;294(28):10760–10772. doi: 10.1074/jbc.RA119.008511

Figure 3.

Figure 3.

Sph3h and PelAh are endo-N-acetylgalactosaminidases with a minimum substrate size of seven residues. Degradation time course of pure oligosaccharides monitored over 30 min by MALDI-TOF MS. Sph3h degradation kinetic of: A, α-1,4-GalNAc 6-mers and B, α-1,4-GalNAc 7-mers. PelAh degradation kinetic of: C, α-1,4-GalNAc 6-mers and D, α-1,4-GalNAc 7-mers. Relative proportion of each ions was calculated and reported here under the color purple for 7-mer; green for 6-mer, blue for 5-mer, red for 4-mer, and yellow for 3-mer.