Figure 6.

PelA_h and Sph3_h differ in their substrate-binding cleft architecture but share catalytic motifs. A, tertiary structure alignment of Sph3_h (PDB 5D5G, purple) with PelA_h. B, transparent surface representation of PelA_h (yellow and blue) and Sph3_h (purple) in the same orientation shows the relative depths of the active site groove. C, alignment of the active site residues of PelA_h (yellow) and Sph3_h (purple) based on the Sph3_h active site motifs shows high identity between the hydrolases around the GalNAc (gray)-binding site of Sph3_h (PDB 5D6T). D, primary sequence alignment of P. aeruginosa PelA_h (PelA_Pa) with homologues from Geobacter metallireducens (PelA_Gm), Ralstonia solanacearum (PelA_Rs), as well as TM1410 and Sph3 (Aspergillus clavatus) done by MUSCLE. Sequence identity to PelA_h is listed based on MUSCLE alignment for the two homologues and Sph3. Sequence identity to TM1410 is based on structural alignment.