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. 2019 Jun 5;294(28):10760–10772. doi: 10.1074/jbc.RA119.008511

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Figure 8. — Difference in electrostatic charge surface predict PelA_h is preferentially able to cleave oligosaccharides containing deacetylated GalNAc. A, electrostatic surface representation of Sph3_h and PelA_h generated using APBS in Chimera. Quantitative electrostatics are colored from red (−15 kT) to blue (+15 kT). B, relative proportions of oligosaccharides obtained from digestion of purified, secreted GAG degradation products with 1 μm Sph3_h or PelA_h. Oligosaccharide products were detected by MALDI-TOF MS. Ions were categorized according to their composition as indicated in the legend. No galactose-homopolymers were detected.