Figure 7.

The hs-Nephrin-Y¹¹³⁹A mutant has dominant-negative effects on the dr-Nephrin function in zebrafish embryos. A, animals untreated or injected with hs-Nephrin-WT, hs-Nephrin-Y¹¹³⁹F, or hs-Nephrin-Y¹¹³⁹A were imaged in bright field at 4 dpf. Additionally, shown are proportions of animals with abnormal glomerular organization patterns. n = 15–40 animals/condition. B, proportion of Tg(wt1a:GFP) animals with normal or abnormal glomerular patterns per denoted treatment were compared examined by a SPIM microscope. n = 17–33 animals/condition. Additionally, shown are the proportion of animals with specific patterns as subgroups in the abnormal glomerular organization group. *, p < 0.05; **, p < 0.0001 versus hs-Nephrin-WT. #, p < 0.05; ##, p < 0.0001 versus hs-Nephrin-Y/F.