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. 2019 May 14;294(28):10789–10806. doi: 10.1074/jbc.RA118.007134

Figure 7.

Figure 7.

X-ray and neutron Guinier Rg and Rxs analyses for IgG2. The X-ray scattering curves of IgG2 are shown for three buffers: A, PBS-50; B, PBS-137; and C, PBS-250 at 20 °C. The concentrations were ∼0.5, 1.0, and 1.5 mg/ml for PBS-50 and PBS-137 and 1.0 and 1.5 mg/ml for PBS-250 from bottom to top. The filled circles between the arrowed data points represent the Q.Rg and Q.Rxs ranges used to determine the Rg and Rxs values. The Q-ranges used for the Rg, Rxs-1, and Rxs-2 values were 0.15–0.28, 0.31–0.47, and 0.65–1.04 nm−1, respectively. The neutron-scattering curves of IgG2 are shown for three buffers: D, PBS-50; E, PBS-137; and F, PBS-250 at 20 °C in 100% heavy water. The concentrations were ∼0.30, 0.59, 1.19, and 2.38 mg/ml for PBS-50; 0.5, 1.0, 2.0, and 3.0 mg/ml for PBS-137; and 0.33, 1.99, and 2.66 mg/ml for PBS-250 from bottom to top. The filled circles between the arrowed data points represent the Q.Rg and Q.Rxs ranges used to determine the Rg and Rxs values. The Q-ranges used for the Rg, Rxs-1, and Rxs-2 values were 0.15–0.28, 0.31–0.47, and 0.65–1.04 nm−1, respectively. Two neutron curves (4 mg/ml in PBS-137 and 0.45 mg/ml in PBS-50) were omitted for clarity.