Figure 10.

Overexpression of CD2AP reduces the lag time before APP degradation induced by starvation. a, effect of CD2AP on the starvation-induced degradation of APP. COS-7 cells were cotransfected with Myc-APP and FLAG-CD2AP and were treated with starvation for the indicated times. The cell lysates were subjected to SDS-PAGE, followed by immunoblotting with anti-Myc for APP and anti-Flag for CD2AP. Actin was the loading control. B, the immunoblot of APP in panel a is redisplayed here after adjustment of the APP signal at time 0 between the presence (+) and absence (−) of CD2AP. c, levels of APP in the presence (+) and absence (−) of CD2AP. Data are expressed as the mean ± S.E. (n = 6; *, p < 0.05, two-way ANOVA analysis, followed by Sidak's multiple comparisons test).