Figure 2.

CD2AP-dependent colocalization of APP with Rab5 and Rab7 visualized by orthogonal projection. X-Y confocal images of HEK293 cells transfected with APP and Rab5 (upper row) or Rab7 (bottom row) in the absence (−) or presence (+) of CD2AP. Rabs were visualized by EGFP fluorescence and APP was immunostained with anti-Myc antibody. The right panels are a higher magnification view of the white box in the merges. The X-Z and Y-Z reconstructions of the white line in the X-Y image are shown at the upper and right side of each panel, respectively. Bars for the low magnification panels, 20 μm; high magnification panels, 5 μm.